Human intestinal cells incubated with activated sludge and lipopolysaccharide express Hsp90b.
نویسندگان
چکیده
Intestinal cells are the first line of defense against toxic substances that may enter the body orally. Some of these substances may emanate from wastewater treatment plants and thus may eventually enter the food chain. In this study, human intestinal Caco-2 cells were incubated with biologically treated activated sludge and lipopolysaccharide (LPS) to determine changes in protein expression and cell monolayer integrity. Caco-2 cells incubated for 3 h with 24-h-aerated activated sludge or 220 microg/ml LPS showed enhanced expression of the heat shock protein 90b (Hsp90b). The results further show that 220 microg/ml LPS is sufficient to irreversibly disrupt tight-junction permeability in 30 min. The Hsp90b expression by the Caco-2 cells incubated with biologically treated activated sludge may be a cellular protective mechanism against LPS-induced stress.
منابع مشابه
Recognition of Betaine as an Inhibitor of Lipopolysaccharide-Induced Nitric Oxide Production in Activated Microglial Cells
Background: Neuroinflammation, as a major outcome of microglia activation, is an important factor for progression of neurodegenerative disorders including Alzheimer's disease and Parkinson's disease. Microglial cells, as the first-line defense in the central nervous system, act as a source of neurotoxic factors such as nitric oxide (NO), a free radical which is involved in neuronal cell death. ...
متن کاملEffects of Dendritic Cell Vaccine Activated with Components of Lieshmania Major on Tumor Specific Response
Background: Dendritic cells (DCs) contribute essentially to the outset and course of immune responses. So in patients with malignancy, there have been considerable interests in use of these cells in different interventions. Objective: To evaluate the impact of Leishmania major’s components on DC maturation and their use as a therapeutic agent against t...
متن کاملNitric Oxide Production and Apoptosis Induction in T Cells through Stimulation of Mesenchymal Stem Cells with Zymosan
Background & Aims: Mesenchymal stem cells (MSCs) are non-hematopoietic multipotant cells, which multiply through attaching to culture plates. Toll-like receptors (TLRs) are inherent immune sensors and regulators of immunomodulatory activities of MSCs. The aim of this study was to investigate the effects of zymosan on stem cell polarization into anti-inflammatory phenotypes through the productio...
متن کاملSalidroside regulates the expressions of IL-6 and defensins in LPS-activated intestinal epithelial cells through NF-κB/MAPK and STAT3 pathways
Objective(s): To reveal the detailed mechanism underlying the functions of salidroside on the inflammation of intestinal epithelial cells during IBD.Materials and Methods: Quantitative real-time PCR was employed to assess the expression of IL-6, IL-10, and α-defensins 5 and 6. ELISA assay was performed to measure the secretion of IL-6 and IL-10. MTT assay was used to determine the cell viabilit...
متن کاملEXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE (iNOS) STIMULATED BY HYDROGEN PEROXIDE IN HUMAN ENDOTHELIAL CELLS
Inducible nitric oxide synthase (iNOS) gene expresses a calcium calmudolin-independent enzyme which can catalyse NO production from L-arginine. The induction of iNOS activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (LPS). Previous studies indicated that all nitric oxide synthases (NOS) activated in human umbilical vein endot...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Environmental sciences : an international journal of environmental physiology and toxicology
دوره 14 1 شماره
صفحات -
تاریخ انتشار 2007